Snapshots in Neuroscience: Synaptic Boutons

This image has been selected to showcase the art that neuroscience research can create.

As described by Dr. van Swinderen and colleagues: The muscles of the larval fruit fly are innervated by two types of motoneurons: 1b and 1s. The synaptic boutons of these neurons (shown in red) can be seen in the image. 1b boutons are larger and have more release sites (shown in green) while 1s boutons are smaller and have fewer release sites. This study found that not all release sites are active in 1b boutons, whereas most are active in the smaller 1s boutons.

The Drosophila neuromuscular junction can be used to study the effects of general anaesthetics on synaptic release. The intravenous anaesthetic propofol decreases the number of active sites proportionally by half in 1b and 1s boutons, without affecting release probability of remaining active sites.

Muscle 6 motor nerve terminals labeled with HRP to identify neurons and nc82 to identify active zones. 1s and 1b boutons are indicated. Scale bar, 4 μm.Images were acquired with a 63x 1.4 NA PlanApo oil immersion objective on a spinning-disk confocal system (Marianas; 3I, Inc.) consisting of an Axio Observer Z1 (Carl Zeiss) equipped with a CSU-W1 spinning-disk head (Yokogawa Corporation of America), ORCA-Flash4.0 v2 sCMOS camera (Hamamatsu Photonics). HRP-Cy3 (Jackson Immunoresearch) was used to label the terminals and Bruchpilot (DSHB) to label synaptic active zones

Read the full article:

Proportional Downscaling of Glutamatergic Release Sites by the General Anesthetic Propofol at Drosophila Motor Nerve Terminals
Shanker Karunanithi, Drew Cylinder, Deniz Ertekin, Oressia H. Zalucki, Leo Marin, Nickolas A. Lavidis, Harold L. Atwood, and Bruno van Swinderen

Category: Snapshots in Neuroscience
Tags: Neuroscience Research, Neuronal Excitability